Part:BBa_K4497011

TEV MESA Part

This part encodes the intracellular protease function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see Part:Bba_K4497017).

Design & Cloning

Design

This part is made of the following components:

• CD 28 transmembrane domain (Part:BBa_K4497004): transmembrane anchor for the MESA system
• Tobacco Etch Virus (TEV) Protease (Part:BBa_K4497005): Protease cleaving proteins the TEV recognition site

Cloning

• G1TA: Part:BBa_K4497017
• G1TEV: Part:BBa_K4497018
• M1TA: Part:BBa_K4497019
• M1TEV: Part:BBa_K4497020
• G2TA: Part:BBa_K4497021
• G2TEV: Part:BBa_K4497022
• M2TA: Part:BBa_K4497023
• M2TEV: Part:BBa_K4497024

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 448
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 448
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 448
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 448
  
• 1000
  COMPATIBLE WITH RFC[1000]